GE28-4083-47

Ab Spintrap^™

Cytiva 28-4083-47

• UNSPSC-Code: 41106500
• NACRES: NA.25

Eigenschaften

• ligand: recombinant protein G lacking albumin-binding region
• Verpackung: pkg of 50 × 100 μL
• Hersteller/Markenname: Cytiva 28-4083-47
• Lagerbedingungen: (20% Ethanol)
• Bettvolumen: 100 μL
• Matrix: highly cross-linked 6% agarose
• Durchschnittliche Teilgröße: 34 μm
• cleaning: 2-9
• working range: 3-9
• Kapazität: >1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.); >1 mg binding capacity(IgG/column)
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Ab SpinTrap^™ are prepacked, single-use spin columns for rapid purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. Designed for small-scale purification of multiple samples in parallel.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Anwendung

Ab SpinTrap^™ is designed for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. In addition, a protocol for immunoprecipitation is included.


The spin columns are also suitable for use in antibody screening experiments.


Ab SpinTrap^™ columns are used together with a standard microcentrifuge and one purification run is performed in less than 20 min.


Ab SpinTrap^™ contains Protein G Sepharose^™ High Performance, which has high Protein binding capacity and is compatible with all commonly buffers used in antibody purification with immobilized Protein G media.


For optimal performance in antibody purification, use Ab SpinTrap^™ together with buffers prepared using Ab Buffer Kit.

Leistungsmerkmale und Vorteile

• Rapid and convenient small-scale purification of IgG.
• Protein G Sepharose^™ High Performance in prepacked spin columns.
• No filtration or centrifugation of different crude samples necessary for antibody purification.
• Classic and crosslink protocols for Protein enrichment (immunoprecipitation).
• Elution conditions formatted for both LC-MS and electrophoresis analysis.
• Elution conditions formatted for both LC-MS and electrophoresis analysis.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

Sepharose is a trademark of Cytiva

SpinTrap is a trademark of Cytiva

Sicherheitshinweise

• Piktogramme: GHS02
• Signalwort: Warning
• H-Sätze: H226
• P-Sätze: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Lagerklassenschlüssel: 3 - Flammable liquids