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GE17-0405-03

HiTrap® Protein G High Performance

Cytiva 17-0405-03, pack of 5 × 5 mL

Synonym(e):

HP columns, Protein G Sepharose column

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About This Item

UNSPSC-Code:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

Verpackung

pack of 5 × 5 mL

Hersteller/Markenname

Cytiva 17-0405-03

Lagerbedingungen

(20% Ehtanol)
(20% Ethanol)

Parameter

42 psi

Bettgröße

16 mm × 25 mm

Bettvolumen

5 mL

Säule ID

16 mm

Größe

Durchschnittliche Teilgröße

34 μm

cleaning

2-9

Arbeitsbereich

3-9

Kapazität

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
125 mg binding capacity(human IgG/column)

Lagertemp.

2-8°C

Allgemeine Beschreibung

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose High Performance for fast, easy separations of IgG from different species and subclasses.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Leistungsmerkmale und Vorteile

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

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Lagerklassenschlüssel

3 - Flammable liquids


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Artikel

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protokolle

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

General instructions for affinity purification using HiTrap columns from Cytiva.

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