GE17-5143-01
HiTrap® Benzamidine Fast Flow
Cytiva 17-5143-01, pack of 5 × 1 mL
About This Item
Empfohlene Produkte
ligand
p-aminobenzamidine
Verpackung
pack of 5 × 1 mL
Hersteller/Markenname
Cytiva 17-5143-01
Lagerbedingungen
(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)
Parameter
42 psi
Bettgröße
7 mm × 25 mm
Bettvolumen
1 mL
Säule ID
7 mm
Matrix
highly cross-linked 4% agarose
Durchschnittlicher Durchmesser
90 μm
cleaning
1-9
Arbeitsbereich
2-8
Lagertemp.
2-8°C
Allgemeine Beschreibung
Anwendung
The medium, Benzamidine Sepharose™ 4 Fast FLow (high sub), is also available as lab packages and is an excellent choice for scale-up. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC™ System.
Leistungsmerkmale und Vorteile
- Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins.
- Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
- HiTrap™ Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
- High binding capacity.
- The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA™ design or FPLC™ System.
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Ähnliches Produkt
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
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Artikel
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
Protokolle
To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin or Factor Xa from the sample.
How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from Cytiva.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
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