Direkt zum Inhalt
Merck
Alle Fotos(1)

Wichtige Dokumente

GE17-5123-10

benzamidin Seph 4Fast Flow H-sub

Cytiva 17-5123-10, pack of 25 mL

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

UNSPSC-Code:
13111000
NACRES:
NA.56

ligand

p-aminobenzamidine

Verpackung

pack of 25 mL

Hersteller/Markenname

Cytiva 17-5123-10

Lagerbedingungen

(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)

Matrix

highly cross-linked 4% agarose

Durchschnittlicher Durchmesser

90 μm

cleaning in place

1-9

Arbeitsbereich

2-8

Lagertemp.

2-8°C

Verwandte Kategorien

Allgemeine Beschreibung

HiTrap® Benzamidine FF (high sub) is an excellent tool for removal of proteolytic activity from a protein.

Anwendung

Benzamidine Sepharose™ 4 Fast FLow (high sub) is an excellent tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as, zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates

Leistungsmerkmale und Vorteile

  • Benzamidine Sepharose™ 4 Fast FLow (high sub) is based on highly cross-linked 4% Agarose enabling high fLow rates without losing binding capacity
  • p-aminobenzamidine (pABA) immobilized on Sepharose™ 4 Fast FLow matrix gives high productivity, and allows easy scale-up.
  • Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins
  • For convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Piktogramme

Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

Leider sind derzeit keine COAs für dieses Produkt online verfügbar.

Wenn Sie Hilfe benötigen, wenden Sie sich bitte an Kundensupport

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Artikel

Purification or Removal of Serine Proteases, such as Thrombin and Trypsin, and Zymogens

This page shows how to Purify or remove serine proteases with a Benzamidine Sepharose 4 Fast Flow, Benzamidine Sepharose 4 Fast Flow from Cytiva.

Principles and Standard Conditions for Different Purification Techniques

This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from Cytiva.

Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

Alle anzeigen

Protokolle

Removal of Thrombin and Factor Xa Using HiTrap® Benzamidine FF (High Sub)

To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin or Factor Xa from the sample.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.