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G7877

Sigma-Aldrich

Glucose-6-phosphat-Dehydrogenase aus Backhefe (S. cerevisiae)

Type VII, ammonium sulfate suspension, ≥200 units/mg protein

Synonym(e):

G-6-P-DH, Zwischenferment

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About This Item

CAS-Nummer:
9001-40-5
EC-Nummer:
1.1.1.49 (BRENDA, IUBMB)
EG-Nummer:
232-602-6
MDL-Nummer:
MFCD00081656
UNSPSC-Code:
12352204
NACRES:
NA.54

Biologische Quelle

bakers yeast

Qualitätsniveau

200

Typ

Type VII

Form

ammonium sulfate suspension

Spezifische Aktivität

≥200 units/mg protein

Mol-Gew.

128 kDa

Methode(n)

cell based assay: suitable

Löslichkeit

water: soluble

Anwendung(en)

diagnostic assay manufacturing

Lagertemp.

2-8°C

Angaben zum Gen

Saccharomyces cerevisiae ... ZWF1(855480)

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Allgemeine Beschreibung

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).

Anwendung

Glucose-6-phosphate dehydrogenase has been used in a study to investigate the effect of dehydroepiandrosterone on cell growth and mitochondrial function in TM-3 cells. Glucose-6-phosphate dehydrogenase has also been used in a study to investigate the incidence of apoptosis and transcript abundance in bovine follicular cells. Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.

Biochem./physiol. Wirkung

Glucose-6-Phosphat-Dehydrogenase katalysiert die Umwandlung von Glucose-6-Phosphat in 6-Phosphogluconolaceton und damit den ersten Schritt des Pentose-Phosphat-Wirkungsweges.

Einheitendefinition

1 U oxidiert 1.0 μmol D-Glucose-6-phosphat zu 6-Phospho-D-gluconat pro Minute bei pH 7.4 und 25°C in Anwesenheit von NADP.

Physikalische Form

Kristalline Suspension in 3.2 M (NH4)2SO4-Lösung, pH 7.0

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

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Xuehuai Shen et al.
General and comparative endocrinology, 177(1), 177-186 (2012-04-03)
Dehydroepiandrosterone (DHEA), a major steroid hormone, decreases with age, and this reduction has been shown to be associated with physical health. In the present study, the effect of DHEA on cell growth and mitochondrial function was investigated using TM-3 cells
D Janowski et al.
Theriogenology, 78(3), 656-669 (2012-05-15)
The close contact and interaction between the oocyte and the follicular environment influence the establishment of oocyte developmental competence. Moreover, it is assumed that apoptosis in the follicular cells has a beneficial influence on the developmental competence of oocytes. The
Gang Zhao et al.
Neurochemistry international, 61(2), 146-155 (2012-05-15)
NADPH derived from glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme of the pentose phosphate pathway, has been implicated not only to promote reduced glutathione (GSH) but also enhance oxidative stress in specific cellular conditions. In this study, the effects of G6PD
Johannes Yayo et al.
Applied and environmental microbiology, 87(9) (2021-02-21)
The native ability of Clostridium thermocellum to efficiently solubilize cellulose makes it an interesting platform for sustainable biofuel production through consolidated bioprocessing. Together with other improvements, industrial implementation of C. thermocellum, as well as fundamental studies into its metabolism, would
Nanette G Vrijenhoek et al.
ALTEX, 39(2), 207–220-207–220 (2022-01-19)
Chemical read-across is commonly evaluated without specific knowledge of the biological mechanisms leading to observed adverse outcomes in vivo. Integrating data that indicate shared modes of action in humans will strengthen read-across cases. Here we studied transcriptomic responses of primary
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Artikel

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protokolle

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

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