G1270

L-Glutamine Synthetase from Escherichia coli

lyophilized powder, 400-2,000 units/mg protein

• Synonym(e): L-Glutamate:ammonia ligase (ADP-forming)
• CAS-Nummer: 9023-70-5
• EC-Nummer: 6.3.1.2 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00131463
• UNSPSC-Code: 12352204
• NACRES: NA.26

Eigenschaften

• Form: lyophilized powder
• Qualitätsniveau: 200
• Spezifische Aktivität: 400-2,000 units/mg protein
• Aufgereinigt durch: affinity chromatography
• Enthält: dithioerythritol as preservative
• Zusammensetzung: Protein, ~5% Lowry
• Löslichkeit: H₂O: soluble 0.95-1.05 mg/mL, clear to hazy
• UniProt-Hinterlegungsnummer: P0A9C5
• Fremdaktivität: ATPase <0.2%
• Lagertemp.: −20°C
• Angaben zum Gen: Escherichia coli K12 ... glnA(948370)

Beschreibung

Allgemeine Beschreibung

L-Glutamine Synthetase from bacteria shows dodecameric structure comprising of 12 active sites. Each active site termed bifunnel, has an ATP and glutamate binding sites. The dodecamer is stabilized by two hexameric rings.

Anwendung

L-Glutamine Synthetase from Escherichia coli has been used in the synthesis of methylglutamine from methylammonium in E coli and in the glutamine synthetase protection activity of human thioredoxin peroxidase enzyme, AOE372.

L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.

Biochem./physiol. Wirkung

L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine. It is a degradative enzyme for glutamic acid.

Nitrogen starvation dictates the expression of the glutamine synthetase (GS) gene in E. coli. GS plays a key role in ammonia assimilation in bacteria. Adenylylation of GS is catalyzed by adenylyltransferase. Adenylylation of GS modulates its catalytic functionality resulting in glutamine limitation in E coli.

Degradative enzyme for glutamic acid

Einheitendefinition

One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37 °C.

Physikalische Form

Contains potassium phosphate, sodium citrate and magnesium acetate buffer salts

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)