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G1041

Sigma-Aldrich

EZBlue™ Gel-Färbungsreagenz

Synonym(e):

protein gel stain, protein stain

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About This Item

UNSPSC-Code:
41105300
NACRES:
NA.32

Methode(n)

protein staining: suitable

Qualitätsniveau

200

Lagertemp.

2-8°C

Allgemeine Beschreibung

Convenient, sensitive, and safe, EZBlue™ Coomassie Brilliant Blue G-250 colloidal protein stain improves protein electrophoresis results while significantly reducing staining time. Conveniently packaged, EZBlue requires no messy weigh-ups or additions of methanol or acid. As a colloidal stain, it reacts only with proteins, not the gel itself. Background staining is reduced, so protein bands can be visualized almost immediately. No destaining step is required, although a water wash may intensify bands and clarify the background. Most impressively, EZBlue is extremely sensitive, detecting as little as 5 ng of protein.

Anwendung

EZBlue Gelfärbungsreagens wird als Färbungsreagens bei der Natriumdodecylsulfat-Polyacrylamidgel-Elektrophorese (SDS-PAGE) eingesetzt.

Leistungsmerkmale und Vorteile

  • Vorgemischte Lösung eliminiert die Zeit und den Arbeitsaufwand für die Färbelösungsherstellung
  • Erhöhte Empfindlichkeit sorgt dafür, dass Proteine in geringen Mengen ??nachgewiesen werden können (schon 5 ng)
  • Schnelle Reaktion reduziert die benötigte Zeit für die Färbung und das Spülen signifikant.
  • Kein Lösungsmittelabfal, Sie sparen Zeit und Geld durch den Wegfall der Gefahrstoffentsorgung

Sonstige Hinweise

Ein gebrauchsfertiger, Brilliantblau G-250 basierter Proteinfarbstoff für einstufigen ultraempfindlichen Nachweis auf Polyacrylamidgelen und PVDF-Membranen.

Rechtliche Hinweise

EZBlue is a trademark of Sigma-Aldrich Co. LLC
SIGMA-ALDRICH is a registered trademark of Merck KGaA, Darmstadt, Germany

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Piktogramme

Health hazardCorrosion
GHS08,GHS05

Signalwort

Warning

Gefahreneinstufungen

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

Zielorgane

Eyes,Central nervous system

Lagerklassenschlüssel

8A - Combustible corrosive hazardous materials

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Rakhi Panda et al.
Journal of agricultural and food chemistry, 63(43), 9629-9639 (2015-10-09)
Many soybean protein products are processed by enzymatic hydrolysis to attain desirable functional food properties or in some cases to reduce allergenicity. However, few studies have investigated the effects of enzymatic hydrolysis on the allergenicity of soybean products. In this
Antibodies to the GABA(B) receptor in limbic encephalitis with seizures: case series and characterisation of the antigen.
Lancaster E, et al.
Lancet Neurology, 9, 67-76 (2010)
AMPA receptor antibodies in limbic encephalitis alter synaptic receptor location.
Lai M, et al.
Annals of Neurology, 65, 424-434 (2009)
Praveen Maurye et al.
Electrophoresis, 38(16), 2060-2068 (2017-04-27)
PAGE is the most widely used technique for the separation and biochemical analysis of biomolecules. The ever growing field of proteomics and genomics necessitates the analysis of many proteins and nucleic acid samples to understand further about the structure and
Nathan L Marsteller et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 141, 111398-111398 (2020-05-22)
Currently no validated animal model is predictive of human responses in ranking purified dietary proteins in the prevalence or potency of food allergy in humans. Since the gastrointestinal microbiota is thought to influence oral tolerance, we hypothesize that a germ-free
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