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Merck

E7510

Sigma-Aldrich

Earle-Salze

Without sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture

Synonym(e):

EBSS

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352207
NACRES:
NA.75

Qualitätsniveau

Sterilität

sterile-filtered

Form

liquid

Konzentration

10 ×

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

endotoxin, tested

Komponenten

glucose: 10.0 g/L
NaHCO3: no
phenol red: 0.11 g/L

Versandbedingung

ambient

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Allgemeine Beschreibung

Earle′s Balanced Salt Solution 10x is a saline solution with physiological pH. It is useful in inducing autophagy and promotes Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) maturation.

Anwendung

Earle′s Balanced Salt Solution 10x has been used:
  • as an additive to the nutritive medium sheep nematode Trichostrongylus colubriformis eggs
  • in the swim-up method to prepare human sperm sample
  • in the steady-state medium to culture bovine primary mammary epithelial cells

Leistungsmerkmale und Vorteile

  • Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance.
  • Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism.
  • When combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism.
  • Provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Rekonstituierung

Supplement with 2.2 g/L sodium bicarbonate at 1×.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Yanli Ren et al.
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Cell death & disease, 12(2), 220-220 (2021-02-28)
Receptor tyrosine kinase (RTK) inhibitors, such as sunitinib and sorafenib, remain the first-line drugs for the treatment of mRCC. Acquired drug resistance and metastasis are the main causes of treatment failure. However, in the case of metastasis Renal Cell Cancer
Zhenlu Li et al.
Cell death & disease, 9(5), 483-483 (2018-05-01)
Autophagy is an essential cytoprotective response against pathologic stresses that selectively degrades damaged cellular components. Impaired autophagy contributes to organ injury in multiple diseases, including ischemia/reperfusion (I/R), but the exact mechanism by which impaired autophagy is regulated remains unclear. Several
Yizhe Sun et al.
Cell death & disease, 8(8), e3031-e3031 (2017-10-20)
Autophagy is a highly inducible system of intracellular degradation that occurs in lysosomes or vacuoles. Transmembrane 74 (TMEM74) has been shown to induce autophagy. However, the mechanism by which TMEM74 stimulates autophagy and the impacts of TMEM74-induced autophagy on tumor
Yuying Fan et al.
Autophagy, 13(1), 41-56 (2016-10-30)
Autophagy is a housekeeping lysosomal degradation pathway important for cellular survival, homeostasis and function. Various disease models have shown that upregulation of autophagy may be beneficial to combat disease pathogenesis. However, despite several recently reported small-molecule screens for synthetic autophagy

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