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D8900

Sigma-Aldrich

Dulbecco Modifiziertes Eagle-Medium/Nährstoffmischung F-12 Schinken

With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Synonym(e):

DME/F-12, 1:1 Mischung

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About This Item

MDL-Nummer:
MFCD00217417
UNSPSC-Code:
12352207
NACRES:
NA.71

Qualitätsniveau

500

Form

powder

Methode(n)

cell culture | mammalian: suitable

Komponenten

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

Versandbedingung

ambient

Lagertemp.

2-8°C

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Anwendung

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. It has been used:

  • as a component of the culture medium for endometrial explants in vitro culture
  • to immerse chitosan-gelatin-glycerol phosphate hydrogels for cytotoxicity evaluation
  • to culture nucleus pulposus (NP) cells to culture human articular chondrocytes for evaluating the toxicity of baicalein

Menge

Formulated to contain 15.6 grams of powder per liter of medium.

Rekonstituierung

Supplement with 1.2 g/L sodium bicarbonate.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Analysenzertifikate (COA)

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Ultrasound in medicine & biology, 45(5), 1306-1315 (2019-02-26)
Cellular aggregates that mimic cell-cell interactions in vitro are essential for biological research. This study introduces a method to form large scaffold-free 3-D aggregates in a clinically ubiquitous cell culture dish using kilohertz-order ultrasound standing wave trapping (USWT). We fabricated
Sang Hwan Kim et al.
PeerJ, 7, e6344-e6344 (2019-02-08)
Here we investigated the expressions of apoptosis-associated genes known to induce programed cell death through mRNA expressions of two matrix metalloproteinases (MMPs) that are involved in the degradation of collagen and basal membrane in luteal cells cultured in the treatment
Elena Ruggeri et al.
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 23(6), 1197-1206 (2017-12-07)
Confocal microscopy was used to image stages of equine zygote development, at timed intervals, after intracytoplasmic sperm injection (ICSI) of oocytes that were matured in vivo or in vitro. After fixation for 4, 6, 8, 12, or 16 h after
Ryosuke Sakumoto et al.
Journal of animal science and biotechnology, 9, 46-46 (2018-06-28)
The aim of the present study was to evaluate CCL8 and CXCL10 expression and its regulatory mechanism in peripheral blood leukocytes (PBLs) at the time of maternal recognition in cows. Blood samples were collected on 14, 15, 16, 17 and
Yi-Ru Chen et al.
Phytotherapy research : PTR, 33(9), 2378-2386 (2019-07-05)
Excessive oxidative stress, which can amplify inflammatory responses, is involved in the pathologic progression of knee osteoarthritis. Diosmin is known to possess a variety of biological functions such as antiinflammatory and antioxidant activities. We therefore demonstrated the chondroprotective potentials of
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Protokolle

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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