CRISPR20V

Lenti CRISPR Universal Non-Target Control#3 Transduction Particles (LV04 vector)

• UNSPSC-Code: 41106609
• NACRES: NA.51

Eigenschaften

• Form: liquid
• Qualitätsniveau: 200
• Verpackung: vial of 200 μL
• Konzentration: (1x10⁶ TU/mL (via p24 titering assay))
• Anwendung(en): CRISPR
• Versandbedingung: dry ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

The Lenti CRISPR Non-Target Control#3 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#3 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types that are not amenable to transfection. The Lenti CRISPR Non-Target Control#3 uses a dual-component system consisting of U6-driven guide RNA (non-target#3) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.

Anwendung

Functional Genomics/Screening/Target Validation

Sonstige Hinweise

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.

Rechtliche Hinweise

CRISPR Use License Agreement

Lentiviral, WPRE, and Evrogen Licenses

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable