CLLS1060

MCF10A CELLS CDC25B (-/-)

human female maMilliporeary glands (Source Disease: Fibrocystic Disease)

• UNSPSC-Code: 41106514
• eCl@ss: 32190102
• NACRES: NA.81

Eigenschaften

• product name: MCF10A CELLS CDC25B (-/-),
• Biologische Quelle: human female mammary glands (Source Disease: Fibrocystic Disease)
• OMIM-Hinterlegungsnummer: 116949
• Lagertemp.: −196°C
• Angaben zum Gen: human ... CDC25B(994)

Beschreibung

Allgemeine Beschreibung

MCF10A CELLS CDC25B (-/-) are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock out modification.

This cell line corresponds to ATCC Catalog No. CRL-10317.

Anwendung

Cell freezing medium-DMSO 1X, Cat. No. C6164

Media renewal changes two to three times per week.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°

The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat No. H6909, to a final concentration of 0.5 ug/mL.

Leistungsmerkmale und Vorteile

These MCF10A cells are adherent with a doubling time of approx. 20 hours

Zinc finger nuclease (ZFN) knockout on chromosome 20p13

Qualität

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Kit-Komponenten auch einzeln erhältlich

• MCF10A CONTROL CELLS 1 vial
• MCF10A CELLS CDC25B (-/-) 1 vial