Direkt zum Inhalt
Merck
Alle Fotos(1)

Key Documents

View All Documentation

CG200

Sigma-Aldrich

cGMP Enzyme Immunoassay Kit, Direct

sufficient for 96 assays

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Verwendung

sufficient for 96 assays

Qualitätsniveau

200

Methode(n)

ELISA: suitable

Versandbedingung

wet ice

Lagertemp.

−20°C

Angaben zum Gen

human ... PRKG1(5592) , PRKG2(5593)

Anwendung

Non-radioactive, competitive immunoassay for the quantitation of total cGMP in tissue and cell cultures. Buffer contains 0.1N HCl that aids in the lysis of cells, inhibits endogenous phosphodiesterases and stabilizes the cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.
Please refer to the attached Protocolfor details.

Nur Kit-Komponenten

Produkt-Nr.
Beschreibung
  • 0.1 M Hydrochloride Acid 1 x 30
  • Acetic Anhydride 1 x 1
  • cGMP-Alkaline Phosphatase Conjugate 1 x 5
  • cGMP EIA Antibody Rabbit Anti-cGMP 1 x 5
  • Cyclic GMP Standard 1 x 0.5
  • Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea
  • Neutralizing Reagent 1 x 6
  • p-Nitrophenyl Phosphate Substrate Solution 1 x 20
  • Plate Sealer 1 ea
  • Stop Solution 1 x 5
  • Triethylamine 1 x 2
  • Wash Buffer Concentrate 1 x 30
Alle anzeigen (12)

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3

Zielorgane

Respiratory system

Zusätzliche Gefahrenhinweise

EUH071

Lagerklassenschlüssel

3 - Flammable liquids

Flammpunkt (°F)

120.2 °F

Flammpunkt (°C)

49 °C

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Lance M Wheeler et al.
Nature communications, 8(1), 1722-1722 (2017-11-25)
Materials with switchable absorption properties have been widely used for smart window applications to reduce energy consumption and enhance occupant comfort in buildings. In this work, we combine the benefits of smart windows with energy conversion by producing a photovoltaic
Yuichiro Kunai et al.
Journal of the American Chemical Society, 139(43), 15328-15336 (2017-10-08)
The concept of electrical energy generation based on asymmetric chemical doping of single-walled carbon nanotube (SWNT) papers is presented. We explore 27 small, organic, electron-acceptor molecules that are shown to tune the output open-circuit voltage (V
Madiha A H Hassan et al.
BMC pharmacology, 5, 10-10 (2005-04-09)
Cardiac hypertrophy is a major risk factor for morbidity and mortality in a number of cardiovascular diseases. Consequently, the signaling pathways that inhibit cardiac hypertrophy are currently receiving much interest. Among them, nitric oxide (NO), signaling via cGMP and cGMP-dependent
Chard, T.
An Introduction to Radioimmunoassay and Related Techniques (1990)
Tijssen, P.
Practice and Theory of Enzyme Immunoassays (1985)

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.