NCI-H69/LX10

96042331, human lung, Not specified

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: NCI-H69/LX10, 96042331
• Biologische Quelle: human lung
• Verpackung: tube of 5 μg 96042331-DNA-5UG; pkg of vial of cells 96042331-1VL
• Wachstumsmodus: Aggregates in suspension
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human small cell lung cancer, drug-resistant

Beschreibung der Zelllinie

The drug-resistant cell line NCI-H69/LX10 has been derived from the parent line NCI-H69 (ECACC No 91091802). It is recommended to culture the cells without drug upon resuscitation; add doxorubicine once growth is fully established.

Anwendung

Drug-resistance studies

Nährmedium

RPMI 1640 + 2mM Glutamine + 1.0 μg/ml doxorubicine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂, 37ºC; resuscitate in drug-free medium and add drug after growth has been established.

Sonstige Hinweise

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