L8

95102434, rat muscle, Myoblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: L8, 95102434
• Biologische Quelle: rat muscle
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Myoblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat Skeletal muscle myoblast

Beschreibung der Zelllinie

The myogenic cell line L8 was isolated by D Yaffe in 1969 by selective serial passage of myoblasts from primary rat skeletal muscle cell cultures (new born, non-in-bred Wistar rats). Subsequently they were recloned by H Blau. L8 cells differentiate into cross-striated myotubes by fusion and express several muscle-specific proteins. The myoblast population will be depleted if cells are allowed to become confluent before sub-culturing. Therefore, sub-cultivation before confluency and periodical re-cloning is necessary. The preparation of a frozen stock at early passage is recommended.

Anwendung

Study of muscle differentiation, expression and analysis of muscle-specific proteins

Nährmedium

DMEM : Medium 199 (4:1) + 2mM Glutamine + 1% CEE (Chick Embryo extract (Seralab)) + 10% Horse Serum (HS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 cells to 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C; sub-cultivate before confluency and reclone periodically.

Sonstige Hinweise

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