RIN-5F

95090402, rat pancreas, Not specified

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: RIN-5F, 95090402
• Biologische Quelle: rat pancreas
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Insulin, L-dopa-decarboxylase
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat islet cell tumour

Beschreibung der Zelllinie

RIN-5F is a secondary clone of the rat islet tumour cell line RIN-m (Sigma Catalogue number. 95071701). Cloning was performed by limited dilution in RPMI 1640, 10%FBS and 20% conditioned medium. The conditioned medium contained growth hormones and prolactin being produced by rat pituitary tumour cells GH3 (Sigma Catalogue number. 87012603). RIN-5F cells secrete insulin, produce high levels of the key APUD enzyme, L-dopa-decarboxylase and contain membrane-bound secretory granules.

Anwendung

Study of pancreatic islet cell biology eg. synthesis, storage and secretion of insulin

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells grow in small islands and do not form a confluent monolayer.

Sonstige Hinweise

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