BC9

95030301, Reactivity: Anti-TNF, bovine

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Mouse x Mouse Hybridoma
• Verpackung: tube of 5 μg 95030301-DNA-5UG; pkg of vial of cells 95030301-1VL
• ImmunoDon: BALB/c mouse spleen
• Myelom: P3X63Ag8.653
• Reaktivität: Anti-TNF, bovine
• Methode(n): cell culture | mammalian: suitable
• Isotyp: IgG1
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse P3X63Ag8.653 x Mouse BALB/c spleen

Beschreibung der Zelllinie

The monoclonal antibody BC9 detects bovine tumour necrosis factor (TNF) in a radioimmunoassay with a detection limit of 24 pg/ml. Bovine recombinant TNF can be neutralised in vitro by addition of BC9. Cytotoxic assays showed a neutralising effect using L929 and WEHI 164 clone 13 cells.

Immunogen

Bovine recombinant TNF-alpha

Anwendung

Detection of TNF in bioassay or radioimmunoassay, in vivo studies (neutralisation of TNF).

Nährmedium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subkultur-Routine

Maintain cultures between 5-6x100,000 cells/ml; 5% CO₂; 37°C.

Sonstige Hinweise

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