149 BLCL human cell line

94031629, IHW Number: SLE

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human blood
• Ethnizität: Caucasian
• Geschlecht: female
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Lymphoblastoid
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice

Beschreibung

Beschreibung der Zelllinie

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA-Profil

Not specified

Serologisches Profil

HLA A: 2 3
HLA C: 5 7
HLA B: 7 44
HLA DR: 2 11
HLA DQ: 1 3

Nährmedium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subkultur-Routine

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Sonstige Hinweise

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Haftungsausschluss

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.