AR42J

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93100618, rat pancreas, Pancreas cells

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: rat pancreas
• Verpackung: tube of 5 μg 93100618-DNA-5UG; pkg of vial of cells 93100618-1VL
• Wachstumsmodus: Semi-adherent aggregates
• Karyotyp: Not specified
• Morphologie: Pancreas cells
• Produkte: Amylase and exocrine enzymes
• Rezeptoren: Gastrin/CCKB, Epidermal growth factor (EGF), Somatostatin
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat exocrine pancreatic tumour

Beschreibung der Zelllinie

Derived from a transplantable tumour of a rat exocrine pancreas. The line is tumourigenic in nude mice, and shows significant secretion of amylase and other exocrine enzymes.

Anwendung

AR42J has been used to obtain a tumor mouse model by inoculating it subcutaneously in a female, athymic nude mouse. It has also been used to perform imaging studies.

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Cells grow in hollow spheroid colonies that can attach loosely. Split cultures 1:2 every 48 hours into fresh flasks, maintain cultures between 1-9x100,000 cells/ml. Adherent cells should be dislodged using 0.2% EDTA. N.B. High cell viability cannot be ex

Sonstige Hinweise

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