JEG3

92120308, human placenta, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: JEG3, 92120308
• Biologische Quelle: human placenta
• Wachstumsmodus: Adherent
• Karyotyp: modal no. 71
• Morphologie: Epithelial
• Produkte: Human chorionic gonadotrophin (hCG), somatomammotrophin
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human choriocarcinoma

Beschreibung der Zelllinie

One of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. Cell line releases chorionic gonadotrophin and somatomammotrophin and progesterone. It is also able to transform steroid precursors to oestrone and oestradiol. JEG3 has the same DNA profile as BeWo (Sigma Catalogue number 86082803); it was established by serial cloning of BeWo .

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin, 5% CO₂; 37°C. Vacuolisation will occur at confluency.

Sonstige Hinweise

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