SW 948

91030714, human colon, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: SW 948, 91030714
• Biologische Quelle: human colon
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Carcinoembryonic antigen (CEA)
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian colon adenocarcinoma

Beschreibung der Zelllinie

Derived from a grade III adenocarcinoma of the colon of an 81 year old Caucasian female. The cells synthesise relatively large amounts of CEA (2,000ng/1,000,000 cells in 21 days).

Anwendung

Tumourigenicity studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 10,11
D16S539: 11,12
D5S818: 11
D7S820: 9,11
THO1: 6,9.3
TPOX: 8,11
vWA: 16,18

Nährmedium

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; No CO₂; 37°C.

Sonstige Hinweise

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