WI 38 Cell Line human

90020107, human lung (fetal), Fibroblast-like

• Synonym(e): WI38 Cells, Wistar Institute-38 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: WI 38 Cell Line human, caucasian fibroblast-like fetal lung cell, 90020107
• Biologische Quelle: human lung (fetal)
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46, diploid except at high passage number
• Morphologie: Fibroblast-like
• Produkte: Collagen
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian foetal lung

Beschreibung der Zelllinie

From normal embryonic lung of female. Have very broad human virus spectra, especially useful for isolation of rhinoviruses. Cells form multilayered membrane when held for long periods at 37°C with periodic pH adjustments. Used in preparation of human virus vaccines.

Anwendung

From normal female embryonic lung (expect collagen production). These cells have a very broad human virus spectra. Particularly useful for the isolation of rhinovirus. Cells from multilatered membrane when incubated for long periods with periodic pH adjustments. Used in the preparation of human virus vaccines.

Subculture: Spit at 70-80% confluency, approx. 1:3 to 1:6, seed at 2-4x10⁴ viable cells/cm². Trypsinize using 0.25% solution, with or without EDTA, 37 °C and 5% CO₂.

Virus studies (rhinoviruses), vaccine production

WI 38 human cell line has also been used to study the cytotoxicity of acrylic based resin compounds and silica nanoparticles.

Biochem./physiol. Wirkung

Finite lifespan. Approx. 50, +/- 10, population doublings. Normal doubling time is 24 hours.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10
D7S820: 9,11
THO1: 8,9.3
TPOX: 8
vWA: 19,20

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan, 50 plus or minus 10 population doublings expected - doubling time 24h.

Hinweis zur Analyse

Recommended culture medium: EMEM (EBSS) + 2 mM L-Glutamine + 1% Non-essential amino acids (NEAA) + 15% FBS

Sonstige Hinweise

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