K562 Cell line human

89121407, from human blood(chronic myelogenous leukemia)

• Synonym(e): K-562 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human blood (chronic myelogenous leukemia)
• Wachstumsmodus: Suspension
• Karyotyp: 2n = 46
• Morphologie: Lymphoblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian chronic myelogenous leukaemia

Beschreibung der Zelllinie

Established from pleural effusion of 53 year old female with chronic myelogenous leukaemia in terminal blast crisis. Population highly undifferentiated and of the granulocytic series. Can be used as a highly sensitive target for the in vitro natural killer assay. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series.

Anwendung

K562 cell line has been used to study the protecting role of cluster of differentiation-39 (CD39) attenuation against DNA damage. It has also been used to study the relevance of a novel sorting approach for separating cells, called “Slice and Dice Sorting”.

Natural killer assay and tumourigenicity studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 8
D16S539: 11,12
D5S818: 11,12
D7S820: 9,11
THO1: 9.3
TPOX: 8,9
vWA: 16

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 100,000 to 1,000,000 cells/ml; 5% CO₂; 37°C.

Sonstige Hinweise

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