LC540

89031604, rat testis, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: LC540, 89031604
• Biologische Quelle: rat testis
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 42
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat Fischer Leydig cell testicular tumour

Beschreibung der Zelllinie

A cell line from a spontaneous transplantable Leydig cell tumour in a Fischer strain rat, reported to secrete steroid, however over a 6 hour period the secretion of oestradiol and testosterone by radioimmunoassay could not be confirmed.

Anwendung

Tumourigenicity studies

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Sonstige Hinweise

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