COS-1 Cell Line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: African green monkey kidney
• Verpackung: tube of 5 μg 88031701-DNA-5UG; pkg of vial of cells 88031701-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Monkey African green kidney, SV40 transformed

Beschreibung der Zelllinie

Derived from CV-1 simian cells transformed by origin-defective mutant of SV40 which codes for wildtype T Ag. Possible host for propagation of pure populations of recombinant SV40 virus. The cells should be handled under laboratory containment level 2.

Anwendung

COS-1 cell line has been used to test the toxicity of various concentrations of iron oxide nanoparticles (for use in medical diagnostics). It has also been used to study the interaction of nanoparticles with their cellular targets.

Virus replication studies: SV40

Nährmedium

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

Subkultur-Routine

Split semi-confluent cultures 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. In order to prevent syncitia formation, do not allow cells become confluent.

Sonstige Hinweise

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