COLO 201

87091201

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human colon
• Wachstumsmodus: Adherent
• Karyotyp: Triploid modal no. 71-78
• Morphologie: Clumps of spherical cells in suspension, cuboidal cells in the monolayer
• Produkte: Carcinoembryonic antigen (CEA) less than produced by COLO 205
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian colon adenocarcinoma

Beschreibung der Zelllinie

COLO 201, COLO 205 and COLO 206 (ECACC Catalogue 93052620) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). At time of isolation, COLO 201 displayed cuboidal morphology but with increasing passage the depositor reported that COLO 201 tends to display predominantly rounded cells in clumps in suspension with cuboidal cells in the monolayer. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 10
D16S539: 12,13
D5S818: 10,13
D7S820: 9,10
THO1: 8,9
TPOX: 11
vWA: 15

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain suspension cells at 3-9x10⁵ cells/cm³. Split sub-confluent cultures (70-80%) of attached cells 1:3 to 1:6 i.e. seeding at 2-3x10⁴ cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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