LoVo Cell Line human

87060101, human colon (supraclavicular lymph node metastasis), Epithelial

• Synonym(e): LoVo Cells
• UNSPSC-Code: 41106514

Eigenschaften

• product name: LoVo Cell Line human, 87060101, from human colon(supraclavicular lymph node metastasis)
• Biologische Quelle: human colon (supraclavicular lymph node metastasis)
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 49, (2n=46)
• Morphologie: Epithelial
• Produkte: Carcinoembryonic antigen (CEA)
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): metastasis
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human colon adenocarcinoma

Beschreibung der Zelllinie

Derived from a metastatic tumour in the left supraclavicular region of a 56 year old male with adenocarcinoma of the colon. The cells produce carcino embryonic antigen (CEA).

Anwendung

Human LoVo cell line is part of the three-dimensional (3D) heterotypic spheroid model developed to assess cancer immunotherapy agents in vitro. It has been used to test the in vitro cytotoxic property and anti-proliferative property of C. lechleri stem bark essential oil.

Tumourigenicity studies

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13,14
D13S317: 8,11
D16S539: 9,12
D5S818: 11,13
D7S820: 10,11
THO1: 9.3
TPOX: 8,9
vWA: 17,18

Nährmedium

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.