WI 38VA13 Subline 2RA

85062512, human lung (fetal), Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: WI 38VA13 Subline 2RA, 85062512
• Biologische Quelle: human lung (fetal)
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46, hyperdiploid, modal no. 73-78
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian foetal lung, SV40 transformed

Beschreibung der Zelllinie

An SV40 transformed derivative of WI 38. The cells are characterised by loss of contact inhibition, unlimited proliferation and the presence of SV40 antigens.

Anwendung

Transformation and virus studies: SV40

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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