NMuMG

94081121, mouse breast, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: NMuMG, 94081121
• Biologische Quelle: mouse breast
• Verpackung: tube of 5 μg 94081121-DNA-5UG; pkg of vial of cells 94081121-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse mammary gland, normal epithelial

Beschreibung der Zelllinie

Initiated from the mammary gland tissue of an adult NAMRU mouse and one of a series of epithelial cell lines derived from normal glandular tissue of mice. The cells exhibit many ultrastructural features of secretory epithelium as well as desmosomes or intermediate junctions, microvilli and tonofibrils. Hemicytes or domes develop when dense monolayers are maintained for several weeks without sub-cultivation. The cells produce benign cystadenomas when inoculated into isogenic mice and are negative for type C oncornaviruses.

Anwendung

NMuMG has been used to compare the actions of insulin like growth factor binding protein 5 (IGFBP-5) with transforming growth factor β 1 (TGFβ1) on epithelial cells. It has also been used to measure the influence of phorbol 12-myristate 13-acetate (PMA) and estradiol on bone morphogenetic protein receptor type 2 (BMPR2) gene expression.

Nährmedium

DMEM + 2mM Glutamine + 10ug/ml Insulin + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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