A2H

85011441, hamster ovary, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: A2H, 85011441
• Biologische Quelle: hamster ovary
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 22
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Hamster Chinese ovary

Beschreibung der Zelllinie

Used for the induction and isolation of nutritionally deficient mutants. A reclone of ′Clone A′ established by Puck (the ′H′ denotes MRC Harwell). Useful for genetic studies due to the low chromosome number.

Anwendung

Genetic studies

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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