UM-UC-6

08090503

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human bladder
• Wachstumsmodus: Adherent
• Karyotyp: Modal number of chromosomes is 51. See reference.
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human transitional cell carcinoma of the bladder

Beschreibung der Zelllinie

UM-UC-6 was obtained by transurethral resection of a bladder transitional cell carcinoma found in a male patient. UM-UC-6 was found to produce tumours in athymic nude mice.

Anwendung

Drug resistance in bladder neoplasms

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11
D13S317: 13,14
D16S539: 9,13
D5S818: 11,13
D7S820: 8,10
THO1: 7
TPOX: 9,11
vWA: 18

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Doubling time approximately 38 hours.

Sonstige Hinweise

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Haftungsausschluss

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.