C6-BU-1

8062563, rat brain, fibroblastic/fusiform

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: C6-BU-1, 08062563
• Biologische Quelle: rat brain
• Verpackung: tube of 5 μg 08062563-DNA-5UG; pkg of vial of cells 08062563-1VL
• Wachstumsmodus: Adherent
• Karyotyp: modal No. of chromosomes is 42
• Morphologie: fibroblastic/fusiform
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat glioma x rat glioma hybrid

Beschreibung der Zelllinie

A 8-bromodeoxybromidine resistant mutant of the rat glioma cell line C6. Found to be tumourigenic in BALB/c nude mice and Wistar rats. Strain rattus norvegicus

Anwendung

Used in the generation of cell hybrids.

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridine.

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C

Sonstige Hinweise

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