BC3H1

86093001, mouse brain, Fibroblast

• UNSPSC-Code: 41106514
• Biologische Quelle: mouse brain
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Acetylcholine
• Rezeptoren: Not specified
• Relevante Krankheit(en): cancer

Eigenschaften

• Produktbezeichnung: BC3H1, 86093001
• Biologische Quelle: mouse brain
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Acetylcholine
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse brain tumour

Beschreibung der Zelllinie

Established in 1974 from a mouse brain neoplasm tumour induced by nitrosoethylurea, possess many properties characteristic of smooth muscle. Since then it has been suggested that BC3H1 is a skeletal line of ectodermal origin that is defective for commitment to terminal differentiation. The two distinct morphologies typical of this cell line are large spheroid cells and smooth-muscle-like cells. The fusiform is the more common.

Anwendung

Acetylcholine receptor studies.

Nährmedium

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subkultur-Routine

Spilt confluent cultures 1:3 to 1:10 i.e. seeding at 1-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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