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Merck

C9205

Sigma-Aldrich

Monoklonaler Anti-GFAP-Antikörper (Glial Fibrillary Acidic Protein, Saures Gliafaserprotein) in Maus hergestellte Antikörper

clone G-A-5, purified from hybridoma cell culture

Synonym(e):

Anti-GFAP

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Konjugat

CY3 conjugate

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

G-A-5, monoclonal

Form

buffered aqueous solution

Speziesreaktivität

human, pig, rat

Methode(n)

direct immunofluorescence: 1:400 using alcohol-fixed, paraffin-embedded rat brain sections

Isotyp

IgG1

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... GFAP(2670)
rat ... Gfap(24387)

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Allgemeine Beschreibung

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 is a purified mouse monoclonal antibody conjugated to Cy3 reactive dye. It is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. GFAP, in the central nervous system, is found in developing, normal, reactive and neoplastic astrocytes and ependymal cells. It is expressed in developing oligodendrocytes in the peripheral nervous system. GFAP is found in some Schwann cells, enteric glial cells, Müller glia of the retina, and the satellite cells of human sensory ganglia. It has been demonstrated in some non-glial cells as well.

Spezifität

Der Antikörper reagiert spezifisch mit GFAP in Immunblotting-Assays und markiert Astrozyten, Bergmann-Gliazellen und Chondrozyten des elastischen Knorpels in immunhistochemischen Färbungen. Der Antikörper reagiert mit gliaspezifischem Antigen in gefrorenen oder alkoholfixierten Gewebeschnitten.
The antibody reacts specifically with GFAP in immunoblotting assays and labels astrocytes, and chondrocytes of elastic cartilage in immunohistochemical staining. The antibody reacts with glial specific antigen in frozen or alcohol-fixed tissue sections.

Immunogen

GFAP aus Schweinerückenmark
GFAP from pig spinal cord

Anwendung

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 has been used in
  • immunohistochemistry
  • immunoblotting
  • immunolabelling,
  • immunocytochemistry
  • immunofluorescence

Biochem./physiol. Wirkung

Glial Fibrillary Acidic Protein (GFAP) is expressed in several glial-related intracranial neoplasms such as astrocytomas and to a variable extent in ependymomas, medulloblastomas, choroid plexus papillomas, gangliogliomas, mixed gliomas, hemangioblastomas, pineocytomas, pineoblastomas, pituitary adenomas, retinoblastomas, oligodendrogliomas and in a small minority of peripheral nerve sheath tumors (schwannomas and neurofibromas). It may also be found in pleomorphic adenomas and ovarian and testicular teratomas.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Rechtliche Hinweise

Cy is distributed under license from Amersham Biosciences Limited.
Cy3 is a trademark of Cytiva

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Antikörper

Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

D B Pettigrew et al.
BMC neuroscience, 2, 9-9 (2001-06-12)
Brain and spinal cord white matter can support extensive axonal growth. This growth is generally constrained to an orientation that is parallel to the longitudinal axis of the fiber tract. This constraint is presumably due to permissive and non-permissive substrates
Sequel of spontaneous seizures after kainic acid-induced status epilepticus and associated neuropathological changes in the subiculum and entorhinal cortex
Drexel M, et al.
Neuropharmacology, 63(5), 806-817 (2012)
Aixa F Rivera-Pagán et al.
PloS one, 10(4), e0125195-e0125195 (2015-04-18)
Excitotoxicity due to glutamate receptor over-activation is one of the key mediators of neuronal death after an ischemic insult. Therefore, a major function of astrocytes is to maintain low extracellular levels of glutamate. The ability of astrocytic glutamate transporters to
Fibroblasts can express glial fibrillary acidic protein (GFAP) in vivo
Hainfellner JA, et al.
Journal of Neuropathology and Experimental Neurology, 60(5), 449-461 (2001)
Expression of vimentin and glial fibrillary acidic protein in central nervous system development of rats
Luo H, et al.
Asian Pacific Journal of Tropical Medicine, 10(12), 1185-1189 (2017)

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