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Sigma-Aldrich

Anti-Clathrin Light Chain antibody, Mouse monoclonal

clone CON.1, purified from hybridoma cell culture

Synonym(e):

Anti-LCB

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About This Item

MDL-Nummer:
MFCD03457551
UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Konjugat

unconjugated

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

CON.1, monoclonal

Form

buffered aqueous solution

Mol-Gew.

antigen 25-36 kDa (doublet)

Speziesreaktivität

human, bovine, mouse, yeast, rat

Konzentration

~2 mg/mL

Methode(n)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using whole rat brain homogenate

Isotyp

IgG2b

UniProt-Hinterlegungsnummer

P09497

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... CLTA(1211) , CLTB(1212)
mouse ... Clta(12757) , Cltb(74325)
rat ... Clta(83800) , Cltb(116561)

Allgemeine Beschreibung

Clathrin, light chain A also known as LCA is a soluble protein composed of heavy and light chain. Clathrin is a protein that plays a major role in the formation of coated vesicles. It coats vesicles from brain, adrenal medulla and non-secreting lymphoma cell line. Clathrin-coated vesicles (CCVs) are major carriers for endocytic cargo and maintain important intracellular trafficking events.

Immunogen

synthetic peptide (amino acids 23-44 in bovine) common to the N-terminus of mammalian clathrin light chains, LCa and LCb, conjugated to KLH. This region is not involved in heavy chain binding and is completely conserved in all mammals.

Anwendung

Monoclonal Anti-Clathrin Light Chain antibody produced in mouse is suitable for the following applications:
  • Immunocytochemistry
  • Immunoprecipitation
  • Microarrary
  • Western blotting at a concentration of 1-2μg/mL using whole rat brain homogenate

Biochem./physiol. Wirkung

Clathrin requires cofactors like ADP-ribosylation factor 6 (ARF6) and adaptin for regulation of receptors. Clathrin light chains (CLCs) are not required for clathrin-mediated endocytosis but plays a critical role for clathrin-mediated trafficking between the TGN and the endosomal system whereas, clathrin heavy chain provides the structural backbone of the clathrin coat. CLCs in mammalian cells function in intracellular membrane trafficking by acting as recruitment proteins for huntingtin-interacting protein 1-related (HIP1R), enabling it to regulate actin assembly on clathrin-coated structures.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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Chen Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(33), 11790-11795 (2008-08-12)
During clathrin-mediated endocytosis, adaptor proteins recognize specific internalization signals on cargo receptors, either recruiting cargos into clathrin-coated pits (CCPs) or initiating clathrin-coat assembly around the cargo molecules. Here, we identify epsin 1, a clathrin-, ubiquitin-, and phospholipid-interacting protein, as a
Delia Bucher et al.
Nature communications, 9(1), 1109-1109 (2018-03-20)
Although essential for many cellular processes, the sequence of structural and molecular events during clathrin-mediated endocytosis remains elusive. While it was long believed that clathrin-coated pits grow with a constant curvature, it was recently suggested that clathrin first assembles to
B M Pearse
Proceedings of the National Academy of Sciences of the United States of America, 73(4), 1255-1259 (1976-04-01)
Coated vesicles have been purified from brain, adrenal medulla, and a nonsecreting lymphoma cell line. A single major protein species, clathrin, with an apparent molecular weight of 180,000, forms the coat of all these vesicles. Peptide mapping suggests that the
J A Ybe et al.
The EMBO journal, 17(5), 1297-1303 (1998-04-18)
Clathrin self-assembly into a polyhedral lattice mediates membrane protein sorting during endocytosis and organelle biogenesis. Lattice formation occurs spontaneously in vitro at low pH and, intracellularly, is triggered by adaptors at physiological pH. To begin to understand the cellular regulation
Daniel G Booth et al.
The EMBO journal, 30(5), 906-919 (2011-02-08)
Kinetochore fibres (K-fibres) of the spindle apparatus move chromosomes during mitosis. These fibres are discrete bundles of parallel microtubules (MTs) that are crosslinked by inter-MT 'bridges' that are thought to improve fibre stability during chromosomal movement. The identity of these
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