C0794

Chitosanase from Streptomyces sp.

buffered aqueous glycerol solution, ≥15 units/mg protein (E^1%)

• Synonym(e): Chitosan N-acetylglucosaminohydrolase
• CAS-Nummer: 51570-20-8
• EC-Nummer: 3.2.1.132 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00211797
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Form: buffered aqueous glycerol solution
• Qualitätsniveau: 200
• Spezifische Aktivität: ≥15 units/mg protein (E^1%)
• Konzentration: ≥0.5 mg/mL
• Löslichkeit: 0.1 M acetate buffer, pH 5.5: soluble
• Lagertemp.: −20°C

Beschreibung

Anwendung

Chitosanase from Sigma has been used for polarized light-stimulated, enzymatic hydrolysis of chitin and chitosan.

Biochem./physiol. Wirkung

Chitosanase catalyzes the endohydrolysis of β-1,4-linkages between D-glucosamine (GlcN-GlcN) residues in chitosan. The enzyme from Streptomyces has been reported to also hydrolyze the GlcNAc-GlcN linkage in partially acetylated chitosan.

Einheitendefinition

One unit will liberate 1.0 μmole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.5 at 37 °C.

Physikalische Form

Solution in 50% glycerol containing 50 mM sodium acetate

Angaben zur Herstellung

Soluble in 10mL of 0.1 M Acetate buffer at pH 5.5

Sonstige Hinweise

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable