B8894
Albumin, acetyliert aus Rinderserum
protease free, for molecular biology
Synonym(e):
Ac-BSA, Acetyl BSA, Acetyliertes BSA
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About This Item
Empfohlene Produkte
Biologische Quelle
bovine
Qualitätsniveau
Qualität
for molecular biology
Produktlinie
BioReagent
Form
solution
Aufgereinigt durch
heat shock fractionation
Enthält
does not contain stabilizer or preservative
Ursprung
USA origin
Konzentration
20 mg/mL in H2O
Methode(n)
FISH: suitable
Verunreinigungen
HIV I and HIVII, HCV and HBsAg, tested negative
UniProt-Hinterlegungsnummer
Fremdaktivität
DNase free
DNase, RNase, NICKase and protease, none detected
NICKase free
Protease free
RNase free
Lagertemp.
−20°C
Angaben zum Gen
bovine ... ALB(280717)
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Allgemeine Beschreibung
Serum albumin is the most extensive plasma protein in the circulatory system. It is produced within the liver and exported as a non-glycosylated protein to the blood plasma. The molecular weight of native serum albumin is around 66 kDa. Native serum albumin is a negatively charged protein.
Acetylation of bovine serum albumin particularly modifies lysine (Lys) residues with elevated frequency, with lesser degrees of modification of serine (Ser) and threonine (Thr) residues. MALDI-TOF mass spectrometry analysis of acetylated BSA has indicated an increased MW of about 3000, compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. This acetylation process also derivatizes similar amino acid residues in any trace contaminating nucleases in the BSA, to inactivate that nuclease activity. This makes acetylated BSA useful in molecular biology applications where trace nuclease activity must be minimized.
Acetylation of bovine serum albumin particularly modifies lysine (Lys) residues with elevated frequency, with lesser degrees of modification of serine (Ser) and threonine (Thr) residues. MALDI-TOF mass spectrometry analysis of acetylated BSA has indicated an increased MW of about 3000, compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. This acetylation process also derivatizes similar amino acid residues in any trace contaminating nucleases in the BSA, to inactivate that nuclease activity. This makes acetylated BSA useful in molecular biology applications where trace nuclease activity must be minimized.
Anwendung
Acetyliert zur Inaktivierung von Nukleasen, die gemeinhin in BSA vorkommen. Acetyliertes BSA sollte nicht als Proteinstandard genutzt werden, da die Acetylierung der Tyrosinreste im Lowry-Assay und in ähnlichen Proteinnachweistests eine Farbentwicklung verhindert.
Acetylated BSA has been used as a component of the reaction mixture for adapter ligation. In addition, this B8894 acetylated BSA product has been used in other applications, such as:
- In cDNA library preparation (Hafner, M. et al., RNA, 17(9), 1697-1712 (2011))
- As a blocking agent with streptavidin beads used for capture of biotinylated RNA (Winz, M.-L. et al., Nat. Protocol., 12(1), 122-149 (2017))
Biochem./physiol. Wirkung
Native serum albumin plays a major role in maintaining the pH and osmotic pressure of the blood. It is also involved in transporting a diverse range of endogenous and exogenous metabolites, such as amino acids, fatty acids, drugs, steroids and metal ions. Native serum albumin can have a role in modulating the levels of Ca2+ and Mg2+ metals in blood.
Leistungsmerkmale und Vorteile
This acetylated BSA product has been tested for the following properties:
- DNase-free / Exonuclease-free
- Nickase-free / Endonuclease-free
- Protease-free
- RNase-free
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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