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Merck

B8520

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)−Biotin antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

rabbit

Qualitätsniveau

Konjugat

biotin conjugate

Antikörperform

IgG fraction of antiserum

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Methode(n)

direct ELISA: 1:200,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300
western blot: 1:400,000-1:800,000

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

Immunoglobulins (Igs) belongs to the immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light comprises of one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is abundant immunoglobulin in human serum.

Immunogen

Purified mouse IgG

Anwendung

Anti-Mouse IgG (whole molecule)-Biotin antibody has been used in
  • immunofluorescence
  • immunoblot
  • direct enzyme linked immunosorbent assay (ELISA)
  • serotyping assay
  • immunohistochemistry

Biochem./physiol. Wirkung

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.

Physikalische Form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Die Dokumentenbibliothek aufrufen

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Clinical and Vaccine Immunology : CVI, 9(6), 1192-1199 (2002)
E P S Conceição et al.
The Journal of endocrinology, 230(2), 263-274 (2016-06-22)
Rats overfed during lactation show higher visceral adipose tissue (VAT) mass and metabolic dysfunctions at adulthood. As both vitamin D and glucocorticoids change adipogenesis, parameters related to metabolism and action of these hormones in the adipocyte can be altered in
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American journal of respiratory and critical care medicine, 199(5), 592-602 (2018-10-20)
Accumulation of B cells and lymphoid follicles (LFs) has been described in chronic obstructive pulmonary disease (COPD) airways, but the functional status of lung B cells remains poorly known. To characterize LFs for expression of IgA, the main mucosal antibody.
Doris Müller-Doblies et al.
Clinical and diagnostic laboratory immunology, 9(6), 1192-1199 (2002-11-05)
This study focused on products of the bovine Mx1 gene as specific markers for acute viral infections. The rationale for this is the fact that viral infections are commonly paralleled by the synthesis, release, and remote action of alpha/beta interferons
Structural and functional composition of the developing retinogeniculate pathway in the mouse
Jaubert-miazza L, et al.
Visual Neuroscience, 22, 661-676 (2005)

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