A8580

Anti-Glutathion-S-Transferase

IgG fraction of antiserum, PBS suspension

• Synonym(e): Anti-GST
• UNSPSC-Code: 12352203
• NACRES: NA.46

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: agarose conjugate
• Antikörperform: IgG fraction of antiserum
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: PBS suspension
• Mol-Gew.: antigen 27.5 kDa
• Darf nicht reagieren mit: porcine, bovine, human, rabbit, rat
• Methode(n): ELISA: suitable; immunoprecipitation (IP): suitable
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... GSTP1(2950)

Beschreibung

Allgemeine Beschreibung

Der Antikörper ist sowohl für native als auch denaturierte Formen von Glutathion-S-Transferase aus Schistosoma japonicum spezifisch. Der Anti-GST-Antikörper kann in verschiedenen Immunassays zur Identifizierung der Expression von GST-Fusionsproteinen eingesetzt werden.

Glutathione-S-transferases (GSTs) are dimeric proteins, which belongs to phase II detoxification enzymes family. The highly polymorphic human cytosolic GSTs are divided into six classes, such as, α, μ, ω, π, θ, and ζ.

Immunogen

Rekombinantes GST aus Schistosoma japonicum wird in E. coli exprimiert.

Anwendung

Immunoprecipitation was performed on NIH 3T3 cells transiently transfected with GST fusion proteins. IP was performed using agarose-linked rabbit anti-GST IgG with mixing for 2 hours at 4 degrees.

Rabbit Anti-Glutathione-S-Transferase (GST)-Agarose antibody can be used for ELISA, immunoaffinity purification and immunoprecipitation assays.

Biochem./physiol. Wirkung

Glutathione-S-transferases (GSTs) can catalyse the conjugation of glutathione (GSH) to various endogenous and exogenous electrophilic compounds. The π and μ classes of GSTs controls the mitogen-activated protein (MAP) kinase pathway. It can also serve as transport proteins.

Physikalische Form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Angaben zur Herstellung

Prepared by coupling cyanogen bromide-activated agarose at 7-8 mg antibody per mL resin volume.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable