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25455

Sigma-Aldrich

4-Chlor-7-nitrobenzofurazan

BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

Synonym(e):

4-Chlor-7-nitro-benzo-2-oxa-1,3-diazol, NBD-chlorid

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About This Item

Empirische Formel (Hill-System):
C6H2ClN3O3
CAS-Nummer:
10199-89-0
Molekulargewicht:
199.55
Beilstein:
614212
EG-Nummer:
233-496-4
MDL-Nummer:
MFCD00005808
UNSPSC-Code:
12352100
PubChem Substanz-ID:
329752738
NACRES:
NA.32

Produktlinie

BioReagent

Assay

≥97.0% (HPLC)

mp (Schmelzpunkt)

97-100 °C
97-99 °C (lit.)

Fluoreszenz

λex 336 nm in methanol
λex 420 nm; λem ~540 nm in ethanol (after derivatization with glycine)

Eignung

suitable for fluorescence

SMILES String

[O-][N+](=O)c1ccc(Cl)c2nonc12

InChI

1S/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H

InChIKey

IGHBXJSNZCFXNK-UHFFFAOYSA-N

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Anwendung

4-Chloro-7-nitrobenzofurazan (NBD-chloride) is a fluorescence assay reagent used to label free sulfhydryls and N-terminals within proteins. NBD-chloride is used as an enzyme inhibitor and as a trapping agent for cysteine sulfenic acid.

Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

H-Sätze

H315,H319

Gefahreneinstufungen

Eye Irrit. 2 - Skin Irrit. 2

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Analysenzertifikate (COA)

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Methods in enzymology, 348, 122-136 (2002-03-12)
C165S AhpC in its sulfenate (Cys-SO-) and presumed thiolate (Cys-S-) forms at pH 7 (pKa for sulfenic acid about pH 6.1) exhibit low extinction absorbance bands around 367 and 324 nm, respectively. Sulfenic acid content of the protein can be
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