DTTP-RO

Thymidin-5′-triphosphat Natriumsalz -Lösung

PCR Grade, sodium salt

• Synonym(e): TTP
• CAS-Nummer: 18423-43-3
• MDL-Nummer: MFCD00080338
• UNSPSC-Code: 12352302
• PubChem Substanz-ID: 329798929

Eigenschaften

• Qualitätsniveau: 100
• Assay: 99% (dNTP)
• Form: solution
• Verwendung: sufficient for 125,000 standard reactions (03732711001); sufficient for 31,250 standard reactions (11969048001); sufficient for 6,250 standard reactions (11934546001)
• Verpackung: pkg of 1,250 μL (11969048001 [100 mM]); pkg of 4 × 1,250 μL (03732711001 [4x100 mM]); pkg of 250 μL (11934546001 [100 mM])
• Hersteller/Markenname: Roche
• Konzentration: <0.9% (dNDP); 100 mM
• Lagertemp.: −20°C
• SMILES String: [Na].CC1=CN(C2CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O2)C(=O)NC1=O
• InChI: 1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
• InChIKey: KAELIXTVSANDSE-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Thymidin-5′-triphosphat-Natriumsalzlösung wird bei DNA-Synthesereaktionen, darunter PCR, DNA-Sequenzierung, cDNA-Synthese und Methoden für molekulares Klonen, verwendet.

PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

dTTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3).

Anwendung

dTTP, PCR Grade, is suitable for many applications where high-quality reagents are required. Such procedures include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.

Qualität

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Physikalische Form

solution of dTTP in water (pH 8.3).

Angaben zur Herstellung

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Sonstige Hinweise

For general laboratory use.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): does not flash
• Flammpunkt (°C): does not flash