11051440001

2′-Desoxyadenosin-5′-triphosphat Natriumsalz -Lösung

=98%, solution, 100 mM, suitable for DNA sequencing, suitable for DNA amplification

• Synonym(e): dATP
• UNSPSC-Code: 41116100

Eigenschaften

• Qualitätsniveau: 100
• Beschreibung: Lithium salt
• Assay: 98%
• Form: solution
• Verwendung: sufficient for 6,250 standard reactions (PCR)
• Verpackung: pkg of 250 μL (25 μmol; 100 mM)
• Hersteller/Markenname: Roche
• Konzentration: 100 mM
• Methode(n): DNA amplification: suitable; DNA sequencing: suitable; DNA synthesis: suitable; PCR: suitable
• Lagertemp.: −20°C
• SMILES String: [Na+].Nc1ncnc2n(cnc12)[C@H]3C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)([O-])=O)O3
• InChI: 1S/C10H16N5O12P3.Na/c11-9-8-10(13-3-12-9)15(4-14-8)7-1-5(16)6(25-7)2-24-29(20,21)27-30(22,23)26-28(17,18)19;/h3-7,16H,1-2H2,(H,20,21)(H,22,23)(H2,11,12,13)(H2,17,18,19);/q;+1/p-1/t5-,6+,7+;/m0./s1
• InChIKey: YJWCICGGRLOGEH-VWZUFWLJSA-M

Beschreibung

Allgemeine Beschreibung

2′-Desoxyadenosin-5′-triphosphat (dATP) besteht aus einer Nukleobase, die an Desoxyribose gebunden ist, und einem 5‘-Hydroxyl an dem Zucker, der an eine Kette aus drei Phosphatresten gebunden ist. dATP wird von Zellen für die Synthese von DNA mittels DNA-Polymerasen verwendet.

dATP is a 100 mM clear, colorless solution of the lithium salt (pH 7).Download the CofA, SDS and IFU s

Anwendung

2′-Desoxyadenosin-5′-triphosphat-Natriumsalzlösung wird für DNA-Synthesereaktionen, darunter PCR, DNA-Sequenzierung und Methoden für molekulares Klonen, verwendet.

Use dATP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.

dATP has been used to resume transcription.

Biochem./physiol. Wirkung

Deoxyadenosine-5-triphosphate (dATP) plays a major role in the initiation of apoptosis in vitro. dATP is considered as an inhibitor of mammalian ribonucleotide reductase, thereby DNA synthesis.

Qualität

Typical analysis:

• ≤98% dATP (HPLC), ≤1.5% dADP (HPLC), ≤0.5% dAMP (HPLC)
• Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀
• DNase activity negative, according to the current Quality Control procedures
• Suitability for PCR

Einheitendefinition

1 μmol dATP = 15.0 A₂₆₀ units

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:

• nucleotide sets (all 4 nucleotides in separate vials)
• ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:

• kits (dNTPs, buffer, and polymerase in separate vials)
• ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): No data available
• Flammpunkt (°C): No data available