11759779001
Roche
BM Chemiluminescence ELISA Substrate (AP)
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Alle Fotos(1)
About This Item
UNSPSC-Code:
12352204
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Verwendung
sufficient for 1,000 tests (ELISA)
sufficient for 600 tests (SEAP)
Verpackung
pkg of 150 mL
Hersteller/Markenname
Roche
Versandbedingung
wet ice
Lagertemp.
2-8°C
Spezifität
The AP assay system is designed for measuring alkaline phosphatase of various sources (e.g. intestine, placenta, milk, bacteria).
Leistungsmerkmale und Vorteile
- Sensitive: Approximately 10fg intestinal alkaline phosphatase can be detected
- High dynamic measuring range: Linear range of more than five orders of magnitude
- Constant light emission: The assay produces a long-lasting light emission instead of a short peak kinetics
Komponenten
1. Alkaline Phosphatase Substrate, CSPD
2. Enhancer, Emerald II
3. Assay Buffer
2. Enhancer, Emerald II
3. Assay Buffer
Prinzip
CSPD is dephosphorylated by alkaline phosphatase (AP). The resulting instable dioxetane anion decomposes and emits light at a maximum wavelength of 477nm. Chemiluminescence-enhancing reagents improve the quantum yield of the excited state by more than 500 fold.
Angaben zur Herstellung
Working solution: Preparation of working solution
The substrate reagent is composed of AP-substrate (bottle 1), Enhancer (bottle 2) and Assay Buffer (bottle 3). Prepare substrate reagent freshly before use. The ratio of the components depends on the application. The substrate reagent is stable at least for one day at 2 to 8 °C.
The substrate reagent is composed of AP-substrate (bottle 1), Enhancer (bottle 2) and Assay Buffer (bottle 3). Prepare substrate reagent freshly before use. The ratio of the components depends on the application. The substrate reagent is stable at least for one day at 2 to 8 °C.
Sonstige Hinweise
For life science research only. Not for use in diagnostic procedures.
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
does not flash
Flammpunkt (°C)
does not flash
Analysenzertifikate (COA)
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