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Merck

SCR011

Sigma-Aldrich

B6-White Murine ES Cell Line

The generation of gene-modified mice, created by homologous recombination in embryonic stem (ES) cells, has become a fundamental tool for analyzing gene function.

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About This Item

UNSPSC-Code:
41106509
eCl@ss:
32011203
NACRES:
NA.81

Biologische Quelle

mouse

Qualitätsniveau

Hersteller/Markenname

B6-White

Methode(n)

cell culture | stem cell: suitable

Aufnahme

sample type: mouse embryonic stem cell(s)

Versandbedingung

liquid nitrogen

Allgemeine Beschreibung

The generation of gene-modified mice, created by homologous recombination in embryonic stem (ES) cells, has become a fundamental tool for analyzing gene function. The influence of genetic background on phenotype has been shown to be an important consideration in selection of a mouse model. Furthermore, the time required to achieve congenic status, which can be 18-24 months has further stimulated demand for targeting in pure inbred strains including C57BL/6 (B6) mice.

Millipore′s B6-White Murine ES cell line is the first commercially available C57BL/6 tyrc-2J albino line that allows for rapid coat-color determination of successful chimerism in the C57BL/6 mouse strain. When B6-White ES cells are injected into C57BL/6 blastocysts, chimeric mice are easily identified by their coat color (a mix of black and white patches), whilst non-chimeric littermates are black. These cells allow for the efficient generation of gene-targeted mice in a pure C57BL/6 genetic background, thus providing more experimental flexibility. Additionally the use of these cells can lower production costs by eliminating the need to maintain an albino blast donor colony, in order to assess chimerism by coat color when targeting in the C57BL/6 strain.

For germline transmission confirmation by coat color, it is recommended that male chimeras are mated with homozygous females of the B6-white strain (strain name: C57BL/6J-Tyrc-2J/J - available from Jackson Labs, stock number 000058).

STRAIN:C57BL/6-tyr(c-2J) albino mice

GENDER: Male

KARYOTYPE: 40XY

Beschreibung der Zelllinie

Embryonic Stem Cells

Anwendung

Research Category
Stammzellforschung

Physikalische Form

Cells are supplied frozen in basal medium supplemented with 10% FCS and 10% DMSO

Lagerung und Haltbarkeit

Place vials in the vapor phase of liquid nitrogen storage immediately upon receipt until it is convenient to proceed to subculture.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2


Analysenzertifikate (COA)

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Li Q Zhang et al.
Cell death & disease, 8(3), e2705-e2705 (2017-03-24)
Nicotinamide phosphoribosyltransferase (NAMPT) is a pleiotropic protein implicated in the pathogenesis of acute respiratory distress syndrome, aging, cancer, coronary heart diseases, diabetes, nonalcoholic fatty liver disease, obesity, rheumatoid arthritis, and sepsis. However, the underlying molecular mechanisms of NAMPT in these
Guangliang Bi et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 32(7), 3583-3596 (2018-02-18)
Although a deficiency of surfactant protein B (SFTPB) has been associated with lung injury, SFTPB expression has not yet been linked with nicotinamide phosphoribosyltransferase (NAMPT), a potential biomarker of acute lung injury (ALI). The effects of Nampt in the pulmonary

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