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PC253L

Sigma-Aldrich

Anti-Calbindin D-28K (Ab-1) Rabbit pAb

lyophilized, Calbiochem®

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.43

Biologische Quelle

rabbit

Qualitätsniveau

Antikörperform

serum

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

lyophilized

Enthält

≤0.1% sodium azide as preservative

Speziesreaktivität

baboon, monkey, rat, human

Hersteller/Markenname

Calbiochem®

Lagerbedingungen

OK to freeze
avoid repeated freeze/thaw cycles

Isotyp

IgG

Versandbedingung

ambient

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... CALB1(793)
rhesus monkey ... Calb1(677721)

Allgemeine Beschreibung

Anti-Calbindin D-28K (Ab-1), rabbit polyclonal, reecognizes the Calbindin D-28K protein in rat hippocampus. It is validated for use in frozen sections and for T cell co-stimulation.
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes the calbindin D-28K protein.
Recognizes the Calbindin D-28K protein in rat hippocampus tissue.

Immunogen

Bovine
purified bovine cerebellum calbindin D-28K protein

Anwendung

Frozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence)

Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, see application references)

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

Undiluted serum.

Rekonstituierung

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.

Hinweis zur Analyse

Positive Control
Rat striatum, hippocampus, or cortex

Sonstige Hinweise

Antibody specificity was examined in the rat striatum, cortex, and hippocampus. Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
Conde, F., et al. 1994. J. Comp. Neurol.341, 95.
Heizmann, C.W. 1993. Acta Neurobiol. Exp.53, 15.
Baimbridge, K.G., et al. 1992. Trends Neurosci.15, 303.
Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci.16, 98.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Empfehlung

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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