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Key Documents

OP07

Sigma-Aldrich

Anti-v-Src (Ab-1) Mouse mAb (327)

liquid, clone 327, Calbiochem®

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About This Item

UNSPSC-Code:
12352203

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

327, monoclonal

Form

liquid

Enthält

≤0.1% sodium azide as preservative

Speziesreaktivität

mouse, human, rat

Hersteller/Markenname

Calbiochem®

Lagerbedingungen

do not freeze

Isotyp

IgG1

Versandbedingung

wet ice

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... SRC(6714)

Allgemeine Beschreibung

Purified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 myeloma cells (see application references). Recognizes the ~60 kDa Src protein.
Recognizes the ~60 kDa v-Src and c-Src proteins in NIH/3T3 and HT1080 cells. May appear as a doublet due to phosphorylation.
This Anti-v-Src (Ab-1) Mouse mAb (327) is validated for use in Immunoblotting, Immunoprecipitation, Frozen Sections, Immunofluorescence, Paraffin Sections for the detection of v-Src (Ab-1).

Immunogen

Epitope: within the SH3 domain of v-Src and c-Src
purified v-Src

Anwendung

Immunoblotting (2.5 µg/ml, see application references)

Immunoprecipitation (1 µg antibody or use Cat. No. OP07A; see application references)

Frozen Sections (not recommended)

Immunofluorescence (not recommended)

Paraffin Sections (not recommended)

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.

Hinweis zur Analyse

Positive Control
NIH/3T3 cells

Sonstige Hinweise

Courtneidge, S.A. and Heber, A. 1987. Cell50, 1031.
Cartwright, C.A., et al. 1985. Mol. Cell. Biol.5,, 2647.
Courtneidge, S.A. 1985. EMBO J.4, 1471.
Whitman, M., et al. 1985. Nature315, 239
Bolen, J.B., et al. 1984. Cell38, 767.
Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci.77, 1311.
Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci.76, 3159.
Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci.75, 2021.
Collett, M.S., et al. 1978. Cell15, 1363.
pp60src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active src kinase (see application references). The agarose conjugate, Cat. No. OP07A, is available separately for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

M S Collett et al.
Proceedings of the National Academy of Sciences of the United States of America, 76(7), 3159-3163 (1979-07-01)
This report extends our previous studies concerning the identification and characterization of a protein from normal cells that is closely related to the avian sarcoma virus (ASV) transforming gene product pp60src. This normal cellular protein, which we have found in
J B Bolen et al.
Cell, 38(3), 767-777 (1984-10-01)
We examine the interaction between polyoma-virus-encoded middle tumor antigen and the cellular src gene product, pp60c-src, using a series of monoclonal antibodies that recognize mammalian pp60c-src. Our results show that infection of mouse cells with transformation-competent strains of polyoma virus
Lisa M Mehlmann et al.
Reproduction (Cambridge, England), 129(5), 557-564 (2005-04-28)
SRC family kinases (SFKs) function in initiating Ca2+ release at fertilization in several species in the vertebrate evolutionary line, but whether they play a similar role in mammalian fertilization has been uncertain. We investigated this question by first determining which
C A Cartwright et al.
Molecular and cellular biology, 5(10), 2647-2652 (1985-10-01)
The polyoma middle tumor antigen (MTAg) associates with the src proto-oncogene product pp60c-src in infected or transformed rodent cells. The tyrosine protein kinase activity of pp60c-src, as measured by in vitro phosphorylation of pp60c-src itself or the exogenous substrate enolase
M Whitman et al.
Nature, 315(6016), 239-242 (1985-05-16)
Polyoma middle-T antigen is required for viral transformation of cultured cells and for tumorigenesis in animals. Like many other transforming gene products, middle-T is bound to the membrane and has an associated tyrosine kinase activity in vitro. This activity seems

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