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Merck

MABN1834

Sigma-Aldrich

Anti-phospho-Filamin-A (Ser2152) Antibody, clone PS2

clone PS2, from mouse

Synonym(e):

Filamin-A, FLN-A, Actin-binding protein 280, ABP-280, Alpha-filamin, Endothelial actin-binding protein, Filamin-1, Non-muscle filamin, phospho-Filamin-A (Ser2152)

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

PS2, monoclonal

Speziesreaktivität

mouse, human

Methode(n)

immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

Isotyp

IgG2bκ

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

phosphorylation (pSer2152)

Angaben zum Gen

human ... FLNA (2316)

Allgemeine Beschreibung

Filamin-A (UniProt P21333; also known as ABP-280, Actin binding protein 280, Alpha-filamin, Endothelial actin-binding protein, Filamin-1, FLN-A, Non-muscle filamin) is encoded by the FLNA (also known as ABP, CSBS, CVD1, FLN, FLN1, FMD, MNS, NHBP, OPD, OPD1, OPD2, XLVD, XMVD) gene (Gene ID 2316) in human. Filamin-A (FLN-A) is an actin-binding protein essential for cytoskeletal rearrangement and cell locomotion. Serine 2152 of FLN-A is a PKA phosphorylation target. FLN-A S2152 phosphorylation is essential for FLN-A-mediated recruitment of signaling proteins, adhesion molecules, and the vesicle-trafficking complex to the peripheral actin rim, so as to stabilize the cortical structure and epithelial membrane. Mutations in FLNA and ARFGEF2 genes cause periventricular heterotopia (PVH or PH), a congenital malformation of cortical development characterized by a reduced brain size and ectopic neuronal nodules along the ventricular neuroepithelium as a result of impaired initiation of neuronal migration. Suppression of either Arfgef2 or Mekk4 results in increased cellular FLN-A level and Ser2152 phosphorylation. In addition, Arfgef2 or Mekk4 deletion causes PVH in knockout mice.

Spezifität

Ser2152 of human and murine Filamin-A isoform 1 (P21333-1 & Q8BTM8-1) corresponds to Ser2144 of human Filamin-A isoform 2 (P21333-2). Equivalent site is not present in murine spliced isoform 2.

Immunogen

Epitope: Filamin repeat 20
KLH-conjugated linear peptide corresponding to the Filamin repeat 20 of human phospho-Filamin-A (Ser2152).

Anwendung

Research Category
Neurowissenschaft
Research Sub Category
Entwicklungsneurowissenschaft
This Anti-phospho-Filamin-A (Ser2152) Antibody, clone PS2 is validated for use in Western Blotting, Immunocytochemistry and Immunofluorescence for the detection of phospho-Filamin-A (Ser2152).
Western Blotting Analysis: A representative lot detected enhanced FLN-A Ser2152 phosphorylation in multiple tissue extracts (liver, heart, brain, and skin) from new born (P0) and E16.5 embryos of Arfgef2/Big2 knockout mice when compared with stage-matched tissue samples from wild-type mice (Zhang, J., et al. (2012). J. Neurosci. 32(36):12619-12629).
Western Blotting Analysis: A representative lot detected cellular FLN-A pSer2152 phosphorylation induction upon serum, forskolin, or EphB2-Fc addition to serum-starved CHP100 human neuroblastoma cells (Zhang, J. et al. (2013). J. Neurosci. 33(40):15735-15746).
Immunocytochemistry Analysis: A representative lot detected cellular FLN-A pSer2152 phosphorylation induction in neuronal progenitors from frontal cortex of E13.5–E14.5 mouse embryos, CHP100 human neuroblastoma cells, and A7, but not FLN-A-deficient M2, human melanoma cells upon forskolin addition (Zhang, J. et al. (2013). J. Neurosci. 33(40):15735-15746).
Immunofluorescence Analysis: A representative lot detected FLN-A pSer2152 immunoreactivity extended to the proliferative zone (PZ) surface at the periventricular heterotopia (PVH) congenital malformation site in the forebrain (FB) of E17.5 MEKK-/- mouse embryos in addition to the FLN-A pSer2152 immunoreactivity in the intermediate zone (IZ) and cortical plate (CP) seen in both wild-type and MEKK-knockout embryos by fluorescent immunohistochemistry (Sarkisian, M.R., et al. (2006). Neuron. 52(5):789-801).

Qualität

Evaluated by Western Blotting in mouse embryonic fibroblast lysate.

Western Blotting Analysis: A 1:125 dilution of this antibody detected Calyculin A-stimulated Filamin-A Ser2152 phosphorylation in mouse embryonic fibroblasts (MEFs).

Zielbeschreibung

~280 kDa observed

Physikalische Form

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Filamin A regulates neuronal migration through brefeldin A-inhibited guanine exchange factor 2-dependent Arf1 activation.
Zhang, J; Neal, J; Lian, G; Hu, J; Lu, J; Sheen, V
The Journal of Neuroscience null
MEKK4 signaling regulates filamin expression and neuronal migration.
Sarkisian, MR; Bartley, CM; Chi, H; Nakamura, F; Hashimoto-Torii, K; Torii, M; Flavell, RA; Rakic, P
Neuron null
Brefeldin A-inhibited guanine exchange factor 2 regulates filamin A phosphorylation and neuronal migration.
Zhang, J; Neal, J; Lian, G; Shi, B; Ferland, RJ; Sheen, V
The Journal of Neuroscience null

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