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MABF80D

Sigma-Aldrich

Anti-MHC class II (I-A/I-E), FITC, clone M5/114 .15.2 Antibody

clone M5/114.15.2, from rat, FITC conjugate

Synonym(e):

H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain (Ii), MHC class II-associated invariant chain, CD74, MHC class II (I-A/I-E), FITC

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

rat

Qualitätsniveau

Konjugat

FITC conjugate

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

M5/114.15.2, monoclonal

Speziesreaktivität

mouse

Verpackung

antibody small pack of 25 μg

Methode(n)

flow cytometry: suitable

Isotyp

IgG2bκ

UniProt-Hinterlegungsnummer

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

mouse ... Cd74(16149)

Allgemeine Beschreibung

The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.

Immunogen

Immunogen information for this antibody is proprietary information.

Anwendung

Research Category
Entzündung & Immunologie
Research Sub Category
Immunoglobuline & Immunologie
Anti-MHC class II (I-A/I-E), FITC, clone M5/114 .15.2 Antibody is an antibody against MHC class II for use in Flow Cytometry.

Qualität

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 μg from a representative lot detected MHC class II (I-A/I-E), FITC in one million C57Bl/6 splenocytes.

Physikalische Form

Purified by size-exclusion chromatography
Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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