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MABF431

Sigma-Aldrich

Anti-CD45.1 Antibody (mouse), PerCP-Cy5.5, clone A20

clone A20, 0.2 mg/mL, from mouse

Synonym(e):

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.44

Biologische Quelle

mouse

Qualitätsniveau

Konjugat

PerCP-Cy5.5

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

A20, monoclonal

Speziesreaktivität

mouse

Konzentration

0.2 mg/mL

Methode(n)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

Isotyp

IgG2aκ

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

mouse ... Ptprc(19264)

Allgemeine Beschreibung

The A20 antibody reacts with mouse CD45.1, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

Immunogen

Immunogen information for this antibody is proprietary information.

Anwendung

Research Category
Entzündung & Immunologie
This mouse monoclonal antibody Anti-CD45.1 (mouse), PerCP-Cy5.5, clone A20 validated for use in IP, IHC & Flow Cytometry for the detection of CD45.1.

Qualität

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 µg of this antibody detected CD45.1 in one million C57Bl/6 or SJL splenocytes.

Physikalische Form

Size Exclusion Chromatography
Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Lagerung und Haltbarkeit

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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