MABE1128

Anti-SETBP1 Antibody, clone 63

clone 63, from mouse

• Synonym(e): SET-binding protein, SEB
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 63, monoclonal
• Speziesreaktivität: human, mouse
• Methode(n): western blot: suitable
• Isotyp: IgG2bκ
• NCBI-Hinterlegungsnummer: NP_001123582
• UniProt-Hinterlegungsnummer: Q9Y6X0
• Versandbedingung: ambient
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... SETBP1(26040)

Beschreibung

Allgemeine Beschreibung

SET-binding protein (UniProt Q9Y6X0; also known as SEB, SETBP1) is encoded by the SETBP1 (also known as KIAA0437, MRD29) gene (Gene ID 26040) in human. SETBP1 is shown to bind the SET nuclear oncogene involved in DNA replication. It is expressed in various tissues with low levels in myeloid and monocytic cells and in CD34+ cells, however, higher expression is observed in myeloid malignancies. Overexpression of SETBP1 promotes leukemogenesis by enhancing full-length SET protein and then impairing the phosphatase activity of the tumor suppressor PP2A through the formation of a SETBP1-SET-PP2A complex. Patients with SETBP1 overexpression have a significantly shorter overall survival, and poor prognosis, particularly in the elderly patients. Mutations in SETBP1 gene are associated with Schinzel-Giedion midface retraction syndrome, which is characterized by severe mental retardation, distinctive facial features, and multiple congenital malformations. (Ref.: Cristobal, I., et al. (2010). Blood 115(3): 615-625).

Spezifität

Clone 63 targets the N-terminal region of human and mouse SETBP1.

Immunogen

GST-tagged recombinant human SETBP1 N-terminal fragment.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Anti-SETBP1, clone 63, Cat. No. MABE1128, is a highly specific mouse monoclonal antibody that targets SETBP1 and has been tested in Western Blotting.

Western Blotting Analysis: A 1:4 dilution of clone 63 hybridoma culture supernatant detected SETBP1 in 30-50 µg of REH human acute lymphoid leukemia cell lysate (Courtesy of Jesper Christensen, Ph.D. and Kristian Helin, Ph.D., University of Copenhagen, Denmark).

Qualität

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SETBP1 in 10 µg of HeLa cell lysate.

Zielbeschreibung

~175 kDa observed. 175.0 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Protein G purified.

Format: Purified

Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1