MABD407

Anti-SIP1 Antibody, clone 6E5

clone 6E5, 1 mg/mL, from mouse

• Synonym(e): Smad-interacting protein 1, SMADIP1, Zinc finger homeobox protein 1b, Zinc finger E-box-binding homeobox 2, SIP1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 6E5, monoclonal
• Speziesreaktivität: human
• Konzentration: 1 mg/mL
• Methode(n): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG2aκ
• NCBI-Hinterlegungsnummer: NP_001165124
• UniProt-Hinterlegungsnummer: O60315
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ZEB2(9839)

Beschreibung

Allgemeine Beschreibung

Smad-interacting protein 1 (SIP1, SMADIP1), also known as Zinc finger E-box-binding homeobox 2 (ZEB2), and Zinc finger homeobox protein 1b (ZFHX1B, ZFX1B), is a transcriptional inhibitor that binds to DNA sequence 5′-CACCT-3′ in different promoters and interacts with activated SMADs. ZEB2 has been shown to repress the transcription of the E-cadherin gene and induce epithelial-mesenchymal transition, a genetic program controlling cell migration during embryonic development and wound healing in vitro. It is suggested that ZEB2 contributes to tumor progression since it’s been demonstrated to protect cells from DNA damage-induced apoptosis. Recent studies have shown that ZEB2 is often observed in the cytoplasm in numerous cancer tissues, implying that its localization may be regulated in normal and tumor tissues. Mutations in this gene are also associated with Mowat-Wilson syndrome (MWIS), also known as Hirschsprung disease-mental syndrome.

Immunogen

Epitope: N-terminus

His-tagged recombinant protein corresponding to the N-terminus of Human SIP1.

Anwendung

Research Category
Stammzellforschung

Research Sub Category
Pluripotente Stammzellen & Stammzellen der frühen Entwicklungsphase

Anti-SIP1 Antibody, clone 6E5 is an antibody against SIP1 for use in western blotting, ICC & IHC.

This antibody has been shown to work in Immunocytochemistry and Immunohistochemistry applications (Oztas et al., 2010).

Qualität

Evaluated by Western Blot in a SK-Hep1 cell lysate. 2ug/ml (1:500 dilution) of this antibody detected SIP-1 in a SK-Hep1 cell lysate.

Zielbeschreibung

~220 kDa observed

Physikalische Form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable